維生素B6對低蛋白日糧下斷奶仔豬生長性能、腸道形態(tài)和基因表達的影響

發(fā)布單位:天津瑞孚農(nóng)牧科技集團有限公司

查看次數(shù)：7405

時間：2020-03-04

維生素B6（VB6）是生物體必需的功能性物質(zhì)，在動物健康中不可或缺。然而，有關(guān)VB6和斷奶仔豬腸道健康互作的研究基本沒有。本試驗目的在于研究低蛋白日糧（18%粗蛋白）下VB6對斷奶仔豬生長性能、腸道健康、炎癥因子和氨基酸轉(zhuǎn)運載體mRNA表達的影響。

試驗選用18頭21日齡斷奶雜交仔豬，初始重7.03±0.17kg，隨機分至3個處理組，VB6添加量分別為0、4、7mg/kg。試驗期14天。

試驗結(jié)果表明處理對生長性能、腹瀉率和生化指標沒有顯著影響。在空腸，VB6不影響腸道形態(tài)和Ki67陽性計數(shù)。4mg/kg VB6組降低了COX-2、IL-10和TGF-β的mRNA表達量（P＜0.05）。日糧添加7 mg/kg VB6提高了SLC7A1、SLC7A6、SLC16A14、SLC38A5的mRNA表達量（P＜0.05），4和7mg/kg VB6組降低了SLC36A1的mRNA表達量（P＜0.05）。在回腸，VB6不影響Ki67陽性計數(shù)，但是顯著降低了絨毛面積（P＜0.05），并有降低絨毛高度的趨勢（P=0.093）。日糧添加4mg/kg VB6顯著提高了IL-1β、TNF-α、COX-2、IL-10和TGF-β的mRNA表達量（P＜0.05）。4和7mg/kg VB6組降低了SLC6A20、SLC7A1、SLC7A6、SLC16A14和SLC38A5的mRNA表達量（P＜0.05）。

結(jié)果表明日糧中添加VB6主要下調(diào)了空腸炎癥因子的表達，上調(diào)了氨基酸轉(zhuǎn)運載體的mRNA表達；而在回腸，上調(diào)了炎癥因子的表達（4mg/kg），降低了氨基酸轉(zhuǎn)運載體的mRNA的表達。這為斷奶仔豬飼喂低蛋白日糧下腸道的發(fā)育提供了相關(guān)參考。

Effects of vitamin B6 on the growth performance, intestinal morphology, and gene expression in weaned piglets that are fed a low-protein diet

Vitamin B6 (VB6), which is an essential functional substance for biosome, plays an irreplaceable role in animal health. However, there are few studies that focus on the correlation between VB6 and intestinal health in weaned piglets. This study was conducted to investigate the effects of VB6 on the growth performance, intestinal morphology, and inflammatory cytokines and amino acid (AA) transporters mRNA expression in weaned piglets that are fed a low crude-protein (CP, 18%) diet. Eighteen crossbred piglets with initial body weights of 7.03 ± 0.17 kg (means ± SEM), weaned at 21-d age, were randomly assigned three diets with 0, 4, and 7 mg/kg VB6 supplementation, respectively. The experimental period lasted 14 days. Our results showed that there were no significant differences in growth performance, diarrhea rate, and biochemical parameters among the three treatments. In the jejunum, dietary VB6 supplementation did not affect the morphology and positive Ki67 counts. Dietary supplementation with 4 mg/kg VB6 decreased the mRNA expression of COX-2, IL-10, and TGF-β (P < 0.05). Dietary supplementation with 7 mg/kg VB6 increased the mRNA expression of SLC7A1, SLC7A6, SLC16A14, and SLC38A5 (P < 0.05) and 4 or 7 mg/kg VB6 decreased SLC36A1 mRNA expression (P < 0.05). In the ileum, VB6 supplementation did not affect positive Ki67 counts but significantly decreased villus area (P < 0.05) and tended to decrease villus height (P = 0.093). Dietary supplementation with 4 mg/kg VB6 had significantly increased the mRNA expression of IL-1β, TNF-α, COX-2, IL-10, and TGF-β (P < 0.05). Dietary supplementation with 4 or 7 mg/kg VB6 had significantly decreased SLC6A20, SLC7A1, SLC7A6, SLC16A14, and SLC38A5 mRNA expression (P < 0.05). These findings suggest that dietary supplementation of VB6 mainly down-regulated inflammatory cytokines and up-regulated AA transporters mRNA expression in jejunum, while up-regulated (4 mg/kg) inflammatory cytokines and down-regulated AA transporters mRNA expression in ileum, which may provide a reference for the intestinal development of weaned piglets that are fed a low-CP diet.

文章來源：豬營養(yǎng)國際論壇
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